简介:Outof1989wildaccessionssowninseedboxesforscreening,only1003wildaccessionswithgoodgerminationwerescreenedagainstbrownplanthopper(BPH),Nilaparvatalugens(St?l)undergreenhouseconditions.Thecollectioncomprisedofaccessionsfrom11wildspeciesandAfricancultivatedrice.ThegermplasmwasscreenedforBPHfollowingstandardseedboxscreeningtechniqueinthegreenhouse.Asmanyas159accessionswereidentifiedasresistantduringtheyear2012basedononeyearscreening.AselectedsetofBPHresistantaccessionswerescreenedagainduring2013.Basedonthetwoyearsscreening,sevenaccessionsofO.nivara(AA),oneaccessionofO.officinalis(CC),sevenaccessionsofO.australiensis(EE),fiveaccessionsofO.punctata(BBandBBCC)andnineaccessionsofO.latifolia(CCDD)wereconfirmedtoberesistanttoBPH.SofarnoBPHresistancegeneshavebeenidentifiedanddesignatedfromO.nivaraandO.punctata,hencethesemayactasnewsourcesofresistance.
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简介:Ininsects,thegustatorysystemplaysacrucialroleinmultiplephysiologicalbehaviors,includingfeeding,toxinavoidance,courtship,matingandoviposition.Gustatorystimulifromtheenvironmentarerecognizedbygustatoryreceptors.Todate,littleisknownaboutthefunctionofgustatoryreceptorsinagriculturalpestinsects.Inthisstudy,weclonedasugargustatoryreceptorgene,N/Gr11,fromthebrownplanthopper(BPH),Nilaparvatalugens(Stal),aseriouspestofriceinAsia;wethenidentifieditsligands,namely,fructose,galactoseandarabinose,bycalciumimagingassay.AfterinjectionofN/Gr11doublestrandedRNA,wefoundthatthenumberofeggslaidbyBPHdecreased.Moreover,wefoundthatN/Gr11inhibitedthephosphorylationofadenosinemonophosphate-activatedproteinkinase(AMPK)andpromotedthephosphorylationofproteinkinaseB(AKT).ThesefindingsdemonstratedthatN/Gr11couldacceleratethefecundityofBPHthroughAMPK-andAKT-mediatedsignalingpathways.Thisisthefirstreporttoindicatethatagustatoryreceptormodulatesthefecundityofinsectsandthatthereceptorcouldbeapotentialtargetforpestcontrol.
简介:对有限波段的混合光谱遥感数据而言,端元的选取及其端元数量对模型精度有重要的影响。随着高光谱平台的发展和数据不断进入各种行业领域,研究人员开始使用高光谱影像进行混合像元分解提取不同组分。但是由于高光谱相邻波段的高度相关性,导致不同地物光谱特征的可分维度没有得到实质性提高,因此在利用LSMA算法进行混合光谱分解时,端元光谱的数量还是受到一定的限制。正是受限于混合像元分解的端元数量,导致LSMA在城市地表组分的分解精度不高,因此在探索如何阐明端元变化对模型精度影响方面有了很多探索性研究,如对端元进行归一化处理;多端元混合像元分解模型(MESAM)得以发展并被广泛应用。