简介:Abstract:Extrusionisanefficientmeasuretoimprovethetextureandphysic-chemicalpropertiesofbrownrice.Thepolynomialdegreetwomodelofextrusiontechnologicalparametersandgelatinizeddegree,waterabsorptionindex,watersolubleindexandmoisturecontentofextrudedmatterwasobtainedbymethodsofsinglefactorandresponsesurfacemethodology,R2=0.9649,0.8745,0.9079,0.8677.Theoptimaltechnoiogica!parametersofbrownriceextrusionwerefiguredoutasfollows:moisturecontentofbrownrice,11.42%,speedofscrew,30rpm,feedingspeed,and20rpm.
简介:摘要:本文介绍了Hoek-Brown强度准则的基本公式以及各个参数的定义和取值,重点叙述了如何利用Hoek-Brown强度准则定量计算岩体的强度参数,包括等效Mohr-Coulomb破坏准则的内摩擦角和粘聚力参数等。此外,本文还选择了某地下交通洞的实际工程案例,依据Hoek-Brown强度准则计算各类围岩的强度参数,并建立二维模型进行有限元分析,最终证明该准则计算隧洞围岩强度参数是合理有效的。
简介:AbstractBackground:Although osteopontin (OPN) is expressed in the liver and pigment gallstones of patients with hepatolithiasis, its role in pigment gallstone formation remains unclear. This study aimed to explore the function of OPN in pigment gallstone formation.Methods:Rats were fed a chow diet (CD) or lithogenic diet (LD) for 10 consecutive weeks; blocking tests were then performed using an OPN antibody (OPN-Ab). Incidence of gallstones and levels of several bile components, OPN, tumor necrosis factor alpha (TNF-α), and cholesterol 7 alpha-hydroxylase (CYP7A1) were analyzed. To determine TNF-α expression in hepatic macrophages and both CYP7A1 and bile acid (BA) expression in liver cells, recombinant rat OPN and recombinant rat TNF-α were used to treat rat hepatic macrophages and rat liver cells, respectively. Chi-square or Fisher exact tests were used to analyze qualitative data, Student t-test or one-way analysis of variance were used to analyze qualitative data.Results:Incidence of gallstones was higher in LD-fed rats than in CD-fed rats (80% vs. 10%, P < 0.05). BA content significantly decreased in bile (t= -36.08, P < 0.01) and liver tissue (t= -16.16, P < 0.01) of LD-fed rats. Both hepatic OPN protein expression (t= 9.78, P < 0.01) and TNF-α level (t= 8.83, P < 0.01) distinctly increased in the LD group; what’s more, CYP7A1 mRNA and protein levels (t= -12.35, P < 0.01) were markedly down-regulated in the LD group. Following OPN-Ab pretreatment, gallstone formation decreased (85% vs. 25%, χ2 = 14.55, P < 0.01), liver TNF-α expression (F = 20.36, P < 0.01) was down-regulated in the LD group, and CYP7A1 expression (F = 17.51, P < 0.01) was up-regulated. Through CD44 and integrin receptors, OPN promoted TNF-α production in macrophage (F= 1041, P < 0.01), which suppressed CYP7A1 expression (F = 48.08, P < 0.01) and reduced liver BA synthesis (F= 119.4, P < 0.01).Conclusions:We provide novel evidence of OPN involvement in pigmented gallstone pathogenesis in rats.
简介:在学习分解因式的过程中,同学们最容易犯的错误就是分解不彻底.所以,在进行分解时,要常常问一问自己:还可以继续分解吗?一、提取公因式后还可以用平方差公式分解
简介:Alightbrownspotted-leafmutantofricewasisolatedfromanethanemethylsulfonate(EMS)-inducedIR64mutantbank.Themutant,designatedaslbsl1(lightbrownspotted-leaf1),displayedlightbrownspotinthewholegrowthperiodfromthefirstleaftotheflagleafundernaturalsummerfieldconditions.Agronomictraitsincludingplantheight,growthduration,numberoffilledgrainsperpanicle,seed-settingrateand1000-grainweightofthemutantweresignificantlyaffected.Geneticanalysisshowedthatthemutationwascontrolledbyasinglerecessivegene,tentativelynamedlbsl1(t),whichwasmappedtotheshortarmofchromosome6.Bydevelopingsimplesequencerepeat(SSR)markers,thegenewasfinallydelimitedtoanintervalof130kbbetweenmarkersRM586andRM588.Thelbsl1(t)geneislikelyanovelricespotted-leafgenesincenoothersimilargeneshavebeenidentifiednearthechromosomalregion.Thegeneticdataandrecombinationpopulationsprovidedwillfacilitatefurtherfine-mappingandcloningofthegene.
简介:棕色的planthopper,Nilaparvatalugens是在整个亚洲的栽培米饭的一个害虫并且用杀虫药剂或抵抗米饭变化被控制。这种开发了抵抗到杀虫药剂的许多班,遗传因子型开发了那对先前是剧毒的抵抗米饭栽培变种。昆虫使用detoxification酶的一间套房,包括保卫自己对的细胞色素P450s,谷胱甘肽S-transferases和carboxyl/cholinesterases种第二等的代谢物和杀虫剂。Roche454-FLX站台上的Pyrosequencing被用来生产补充存在Sanger的数据集定序的一个实质的表示顺序标签(EST)在GenBank的EST。一个总数78959读与37392公开地可得到的SangerEST被相结合;这些集合了进8911contigs和10620单条。分发的分析试验有为生物进程和分子的函数的基因本体论的唯一的基因(拖船)建议454并且SangerEST汇编N是广泛地代表性的。lugenstranscriptome。planthoppertranscriptome被发现包含编码P450s,九个编码谷胱甘肽S-transferases和这些的26编码carboxyl/cholinesterases和许多的31条拖船的褐通常认为地涉及xenobiotics的detoxification。AgilenteArray站台被用来构造为19000个unigene序列与探针占据的oligonucleotidemicroarray,包括知道编码detoxification酶的所有那些。在这研究开发的genomic资源将对学习这个庄稼害虫的社区有用并且将帮助阐明位于杀虫药剂抵抗和planthopper改编下面到抵抗米饭栽培变种的分子的机制。
简介:TheimpactofBrownCarbon(BrC)toaerosollightabsorptionhasbeenpaidmoreattentionrecentlyandtherearealargenumberofstudiesshowingthattheinfluenceofBrConradiativeforcingshouldnotbeignored.BrCalsoactsasanimportantcomponentofhazepollutionwhichisoccurringfrequentlyinWuhan,China.Therefore,itisessentialtoestimatetheiropticalproperties,composition,andmassconcentration.Consideringmosthazepollutionhappensduringthecoldesttime,weretrievedBrCcolumnarcontentduringwinterinWuhanforthefirsttime.OurmethodbasesonthefactthatBrCshowedthestrongspectraldependenceonUV-lightabsorption.Usingthismethod,wefoundthatBrCmakesupthesmallproportionsoftotalaerosolvolume(lessthan10%).Inthewinterof2011,weretrievedthedaily-averagedcolumnar-integratedmassconcentrationofBrConcleardayis4.353mg/m2whilethatofhazedayis12.750mg/m2.Accordingtothesensitivitystudy,wefoundthattheresultshighlyreliedontheassumedaerosolrefractiveindex.Toreducetheuncertaintyofthisapproach,weneedtogainabetterunderstandingofthetemporalvariabilityoftheradiationabsorbingcomponentsoftheseaerosolsinthefuture.
简介:布朗水藻(Chromista,Ochrophyta,Phaeophyceae)是多细胞的水藻的一个大组在海洋生态系统和生物多样性起重要作用。然而,为学习他们新陈代谢的特征妨碍了的种系发生的进化和小说的差的分子的库在这个领域里进行。在这研究,我们在中国定序棕色的水藻的18主要种类的denovotranscriptome,盖住六份订单和七个家庭,用高产量的定序站台IlluminaHiSeq2000。从这的transcriptome数据18种类和Ectocarpussiliculosus和Phaeodactylumtricornutum的公开可得到的染色体数据,我们识别了108原子产生orthologous基因并且基于一个multigene方法在这些棕色的水藻之中澄清了种系发生的关系。这些棕色的水藻能被分开成二clades:CladeIshigeales-Dictyotales和CladeEctocarpales-Laminariales-Desmarestiale-Fucales。前者在种系发生的树的底,当后者被划分成二根树枝时,显示它的早分叉,与从订单Ectocarpales,Laminariales,和Desmarestiale分叉的顺序Fucales。在我们分类有争议的种类的分析,马尾藻类海草fusiforme和Saccharinasculpera被发现是仔细与类马尾藻类海草和Saccharina有关,分别地当Petalonia扁带显示出可能的关系到类Scytosiphon时。学习为棕色的水藻的种系发生的分类提供了分子的证据。