简介:目的:针对较大翼状胬肉,采用胬肉切除、角膜缘干细胞移植联合全角膜羊膜覆盖术,或胬肉切除和角膜缘干细胞移植术后配戴绷带型角膜接触镜进行治疗,观察两种方法的临床效果。方法:将侵及瞳孔区的较大翼状胬肉40眼随机分成两组,一组行翼状胬肉切除、角膜缘干细胞移植术联合全角膜羊膜覆盖术(羊膜组),另一组行胬肉切除和角膜缘干细胞移植术后配戴绷带型角膜接触镜(绷带镜组),每组各20眼。比较两组患者手术时间,以及术后第l、3d,1、3wk眼部舒适程度和角膜愈合情况与随访复发情况等。结果:平均手术时间:羊膜组为61.4±5.2min,绷带镜组为34.5±2.7min,绷带镜组手术时间明显缩短,两组差异有统计学意义(P<0.05)。两组患者的眼部舒适度在术后第Id无明显差异,但在术后第3d和术后lwk,绷带镜组患者的眼部舒适度明显优于羊膜组(P<0.01),至术后3wk时两组间差异无统计学意义(P>0.05)。术后3wk时两组患者的角膜愈合率评分分别为0.85±0.18分和1.15±0.18分,两组差异无统计学意义(P=0.25)。羊膜组有1眼胬肉复发。结论:在侵及角膜的较大翼状胬肉治疗中,胬肉切除和角膜缘干细胞移植术后联合全角膜羊膜覆盖或术后配戴绷带型角膜接触镜均有助于缓解眼部不适症状和促进角膜创面修复,使用绷带型角膜接触镜患者更舒适,治疗更便捷。
简介:·AIM:Toinvestigatetheantifibroticeffectoffreeze-driedbilayeredfibrin-bindingamnioticmembraneontrabeculectomyinarabbitmodel.·METHODS:Twenty-fourJapanesewhiterabbitswererandomizedintothreegroups:theexperimentalgroup(oculartrabeculectomyincombinationwithfreeze-driedbilayeredfibrin-bindingamnioticmembranetransplantation),thecontrolgroup(oculartrabeculectomyincombinationwithnaturalbilayeredfibrin-bindingamnioticmembrane)andtheblankgroup(singletrabeculectomy).Clinicalobservation,hematoxylin-eosinstaining,Massionstaining,real-timePCRandimmunohistochemistryforα-SMAwereperformedondays7,14,21and30followingsurgery.·RESULTS:Statisticaldifferenceswerenotedinsurvivalanalysisandintraocularpressure(IOP)amonggroupsondays7,14,21and30followingsurgery.Histology,immunoh-istochemistryandreal-timePCRfurtherdemonstratedthattrabeculectomyincombinationwithfreeze-driedbilayeredfibrin-bindingamnioticmembraneresultedingoodwoundhealingandnoscarformation.·CONCLUSION:Self-madefreeze-driedbilayeredfibrin-bindingamnioticmembranemayinhibittheformationofscarringinglaucomaaftertrabeculectomy.·
简介:AIM:Toinvestigatetheantifibroticeffectofthefreeze-driedbilayeredfibrin-bindingamnioticmembraneasadrugdeliverysystemonglaucomasurgeryinrabbitmodel.Theaimofthisstudywastoprepareanovellocaldeliverysystemforthesustainedandcontrollablereleaseof5-Fu.METHODS:Twenty-fourJapanesewhiterabbitswererandomizedintothreegroups:theexperimentalgroup(oculartrabeculectomyincombinationwith5-Fuloadedfreeze-driedbilayeredfibrin-bindingamnioticmembranetransplantation),thecontrolgroup(oculartrabeculectomyincombinationwith5-Fu)andtheblankgroup(singletrabeculectomy).HEstaining,massionstainingandimmunohistochemistryforα-SMAwereperformedondays7,14,21and30followingsurgery.Theconcentrationof5-Fuinrabbitaqueoushumorwasexaminedbyhighperformanceliquidchromatography(HPLC)3daysafterthesurgery.RESULTS:Statisticaldifferenceswerenotedinintraocularpressureamonggroupsonday7,14,21and30followingsurgery.Histologyfurtherdemonstratedthattrabeculectomyincombinationwithfreeze-driedbilayeredfibrin-bindingamnioticmembraneyieldedwellwoundhealingandnoscarformationandwasbeneficialforlongtermeffect.CONCLUSION:HPLCshowedagoodslow-releaseeffectwithfreeze-driedbilayeredfibrin-bindingamnioticmembrane.
简介:目的观察透明角膜切口白内障超声乳化吸除人工晶状体植入术联合翼状胬肉切除羊膜移植术的治疗效果.方法回顾性分析我院2009年2月至2013年3月所行透明角膜切口白内障超声乳化吸除人工晶状体植入术联合翼状胬肉切除羊膜移植术.结果34例(36只眼)老年性白内障并发不同程度的翼状胬肉,行透明角膜切口超声乳化白内障吸除及人工晶状体植入术联合翼状胬肉切除及羊膜移植术,手术后角膜创面4~5d愈合,无1例胬肉复发,无新生血管生长及瘢痕组织增生现象.术后视力33只眼视力>0.3,脱残率91.7%.结论超声乳化白内障吸除人工晶状体植入术联合翼状胬肉切除羊膜移植术是安全有效的,可操作性强.
简介:目的分析前房穿刺术在急性闭角型青光眼急性发作期药物不能控制眼压中的疗效及其并发症情况.方法对21例22眼急性闭角型青光眼急性发作患者在应用常规降眼压药物治疗未能有效降压后,采取表麻下手术,显微镜下进行前房穿刺术放液,观察眼压控制以及并发症情况.结果术前眼压均≥50mmHg,所有患者经穿刺放液后,症状缓解,眼压下降,平均为21.5mmHg,视力提高,角膜水肿及睫状充血明显好转,8例患眼术后次日复查眼压复上升,于原穿刺切口再放液,眼压控制后予以相应手术,术后恢复良好.结论前房穿刺术是治疗急性闭角型青光眼急性发作期高眼压持续状态的有效方法,损伤小,反应轻,并发症少,为急性闭角型青光眼进一步治疗创造了条件,并且能够改善其预后.