简介:Aridsoilswherewaterandnutrientsarescarceoccupyover30%oftheEarth’stotalsurface.However,themicrobialautotrophyintheharshenvironmentsremainslargelyunexplored.Inthisstudy,theabundanceanddiversityofautotrophicbacteriawereinvestigated,byquantifyingandprofilingthelargesubunitgenesofribulose-1,5-bisphosphatecarboxylase/oxygenase(RuBisCO)formI(cbbL)responsibleforCO2fixation,inthearidsoilsunderthreetypicalplanttypes(Haloxylonammodendron,Cleistogeneschinensis,andReaumuriasoongorica)inNorthwestChina.Thebacterialcommunitiesinthesoilswerealsocharacterizedusingthe16SrRNAgene.Abundanceofred-likeautotrophicbacteriarangedfrom3.94×105to1.51×106copiesg-1drysoilandthoseofgreen-likeautotrophicbacteriarangedfrom1.15×106to2.08×106copiesg-1drysoil.Abundanceofbothred-andgreen-likeautotrophicbacteriadidnotsignificantlydifferamongthesoilsunderdifferentplanttypes.TheautotrophicbacteriaidentifiedwiththecbbLgeneprimerweremainlyaffiliatedwithAlphaproteobacteria,Betaproteobacteriaandanunculturedbacterialgroup,whichwerenotdetectedinthe16SrRNAlibrary.Inaddition,25.9%and8.1%ofthe16SrRNAgeneswereaffiliatedwithCyanobacteriainthesoilsunderH.ammodendronandR.soongorica,respectively.However,noCyanobacteria-affiliatedcbbLgenesweredetectedinthesamesoils.TheresultssuggestedthatmicrobialautotrophicCO2fixationmightbesignificantinthecarboncyclingofaridsoils,whichwarrantsfurtherexploration.
简介:DREB类转录因子是一类植物中特有的,在非生物逆境胁迫中起重要作用的调控因子。本研究以割手密为材料,采用同源基因克隆的方法克隆获得2个DREB2类转录因子基因SsDREB2-1和SsDREB2-2(GenBank登录号分别为KU963264和KU963265)。序列分析表明,这2个基因的长度分别为814bp和709bp,分别编码262和227个氨基酸;预测其蛋白质分子量分别为28.66kD和24.95kD,等电点(PI)分别为5.42和6.47。氨基酸亲水/疏水性分析表明,这2个转录因子属于亲水性蛋白。多序列比对分析表明,两基因序列的相似性为98.7%。原核表达分析表明,这2个基因编码区能正常表达出目的蛋白,说明得到的这2个基因为功能基因而非假基因。
简介:通过莰-2-基脲与芳香醛缩合制得中间体亚胺后再与仲亚磷酸酯反应得一系列标题化合物A或由莰-2-基脲、芳香醛和仲亚磷酸酯一锅反应得到A.中间体亚胺与标题化合物的化学结构经IR、1HNMR、31PNMR、MS及元素分析确证.生物测定结果表明所合成的化合物A对水稻发芽表现较好的调节活性.
简介:TheimpactsofelevatedtemperatureandCO2onyoungsilverbirch(BetulapendulaRoth)saplingsafter0,25,50or75%artificialdefoliationwereassessedbymeasuringplantheightanddrymassofabovegroundcompartmentsandrootsandvariousmorphologicalandphysiologicalvariables.Defoliationeitherincreasedordecreasedplantgrowthdependingontheseverityofdamageandtheclimatictreatment.At21Cand400mgL-1CO2,defoliatedplantswerenotabletocompensateforthelostfoliage,butgrowthcompensationandadaptationtothechangedconditionsweregreater;growthofyoungdefoliatedsilverbirchsaplingsincreased,whichledtoincreasedheightandatendencytoenhancefinalabovegroundandrootbiomassandleafnitrogenandcarboncontentcomparedtothenondefoliatedcontrols.Nevertheless,theshort-termeffectofthedifferentclimaticconditionsdidnotresultinasignificantovergrowthofdefoliatedplants.AslightincreaseintemperatureandCO2werethemostacceptableconditionsfordefoliatedplants;however,a4CincreasewithcorrespondinglyhigherCO2wasmorestressfulasshownbylessgrowthinheightandbiomassallocationtoleaves,stemsandroots.Thefindingsfromthepilotexperimentaremoreapplicabletoyoungbirchtrees,butstressonyoungtreesmaybereflectedinfuturetreegrowth.
简介:Biochar是生物资源热分解获得并且考虑了碳隐遁的一个平均数的一个充满碳的产品。在这研究,从食物与农业科学的学院的农场的沙的石灰质的土壤,绍德国王大学,沙特阿拉伯,与也被修改Conocarpus直立的L的木质的废物。(CW)或biochar(BC)以0的率(控制)从CW生产了,10,30和50gkg−1。土壤pH,溶解的器官的碳(文档),微生物引起的生物资源碳(MBC),公司2排放和新陈代谢的商上的修正案的效果(沙的石灰质的土壤的qCO2)在一个60-d孵化实验被学习。结果证明CW的增加与控制和BC的增加相比在土壤pH导致了重要减少。公司2-C排放率比孵化时间什么时候进行在孵化的开始几天内是更高的。特别以更高的率,从与CW修改的土壤的累积公司2-C排放更高(近似到6褶层的3-)与BC从控制和土壤比那修改了。修改BC的土壤证明因为与非修改的土壤,而是累积公司2-C的增加相比排放不在孵化的60d以后是重要的,公司2-C排放的重要增加在孵化的第一天期间评价。在另一方面,CW应用比控制和BC应用导致了更加更高累积的公司2-C排放,MBC和文档。与0白天的异常(在孵化的1h以后),导致的CW和BC应用程序作为与控制相比降低qCO2的值。力量功能运动模型令人满意地描述了累积公司2-C排放。通常,公司2排放的最低价值与BC在土壤被观察,建议到公司2排放的BC的贡献作为与CW的相比很小。