简介:为在米饭的壳硅内容的QTLqHUS6以前位于米饭染色体6的短手臂。由使用在在一个isogenic背景怀有qHUS6的RM587RM19784区域分离的一张F2:3人口,为壳硅内容的二QTL被检测,哪个qHUS6-1在对着丝点近似的区域位于远侧的区域和qHUS6-2。在目标区域带小异质接合的片断的三米饭植物被选择,哪个二盖住qHUS6-1区域,其它盖住qHUS6-2区域。三张F2:3人口分别地从三植物的selfed种子被导出。印射的QTL用在qHUS6-1区域分离的二张人口被执行,并且qHUS6-1被标记RM510和RM19417限定到147.0-kb区域flanked。有在qHUS6-2区域的不同genotypic作文的五组F3线从另外的F2:3人口被选择。二QTL用双向ANOVA,qHUS6-2a位于RM19706RM19795和qHUS6-2b在间隔RM314RM19665定义的间隔被分开。
简介:Moleculardesignbreedingisoneofstraightforwardapproachestobreakyieldbarriersinrice.Inthisstudy,GW6geneforgrainlengthandwidthfromBaodaliwastransferredintoanindicarecurrentparent9311andajaponicavarietyZhonghua11(ZH11)usingmarker-assistedbackcross(MAB).Oneandthreeintrogressionlineswereselectedforphenotypicanalysisfrom9311andZH11geneticbackgrounds,respectively.SSL-1,animproved9311nearisogeniclinewithGW6performed11%,19%and6.7%higherofgrainlength,1000-grainweightandsingleplantyield,respectively,ascomparedwith9311.AllthethreeimprovedZH11-GW6lines,R1,R2andR3,hadmorethan30%increaseingrainweightandabout7%higheringrainyield.SeedplumpnessofR1,R2andR3wasimprovedsynchronouslybecausethethreeZH11-GW6linescontainedGIF1(GrainIncompleteFilling1),adominantgrainfillinggene.Thus,GW6hashighpotentialinincreasingtheyieldofinbredlinesthroughMAB,makingitanimportantgeneticresourceinsuperhybridricebreeding.ThisstudyprovidesinsightsintheutilizationofGW6forlargegrainandhighyieldricebreedingviamoleculardesignbreeding.
简介:盆栽试验被进行学习不同的氮应用程序时间的效果(在期间直到ering或孕穗期)与一样,氮在颖果开发和米饭变化Yangdao6的谷物质量评价。增加的氮肥(脲),特别在孕穗期期间适用,能显然增加milled,雪白大米率和蛋白质与控制(没有氮申请)相比在米饭满足谷物,并且减少白垩的谷物率和直链淀粉内容。而且,增加的氮肥显著地影响了颖果发展并且当氮适用在期间时,提高了粒重直到ering和孕穗期,特别在孕穗期期间。在颖果开发期间,增加的氮肥适用在期间直到ering和孕穗期能显然减少全部的淀粉和直链淀粉内容,然而并非显然为在米饭的胶淀粉内容谷物。氮肥的增加的顶肥,特别在孕穗期期间适用,在amyloplasts和proteinoplasts的发展和结构的有的重要效果。也就是说,它能改变分发,数并且amyloplasts和proteinoplastsin塑造特别在谷物腹部的内乳房间。与控制相比,amyloplasts和proteinoplasts的安排各是更靠近的,与更多的数字,更高的密度和更少的间隙星际ohter。而且,大多数amyloplasts在增加的氮肥水平下面显示出多面体。
简介:有低glutelin内容的瑞斯作为为肾失败影响的病人的功能的食物合适。在米饭的低glutelin内容基因Lgc1有在二高度类似的glutelin基因GluB4和GluB5之间的3.5-kb删除,它在染色体2的短手臂上定位。在低glutelin内容米饭改进选择效率繁殖,指定为InDel-Lgc1-1和InDel-Lgc1-2的二个分子的标记被开发检测低glutelin内容基因Lgc1。双PCR察觉显示二个标记的联合使用能容易把Lgc1的遗传型与不同米饭变化区分开来。作为一种简单、便宜的技术,因此,分子的标记能广泛地被用来与Lgc1基因识别不同变化并且在低glutelin内容米饭的帮助标记的选择适用。
简介:Aricepopulationconsistingof90TN1/GulyiguF3lineswasemployedtoanalyzethelinkagebetweenDNAmarkersandanewgeneWbph6(t)conferringresistancetowhitebackedplanthopper,Sogatellafurcifera.Byusingthemappingapproachofbulkedextremesandrecessiveclass,Wbph6(t)wasmappedontotheshortarmofchromosome11withageneticdistanceof21.2cMtoSSLPmarkerRM167.
简介:与较普通活字大一倍的杂交稻Yangliangyou6(YLY6)和Liangyoupeijiu(LYPJ),andthree线杂交稻Shanyou63(SY63)作为与谷物充满联合的材料,来源,水池和流动特征被调查。种子背景率,谷物充满度和YLY6和SY63的谷物产量比LYPJ的那些显著地高。在YLY6和SY63的灰煤杆和鞘的事的出口和转变百分比比LYPJ的那些显著地高。在谷物的蔗糖synthase,腺苷diphosphoglucosepyrophosphorylase,淀粉synthase和淀粉分叉酶的活动比为LYPJ为YLY6andSY63是更高的,并且很显著地与充满率,吝啬的谷物充满率,谷物充满度和粒重的最大的谷物被相关。每维管束的区域和YLY6和SY63的韧皮部的小穗状花小穗数字,谷物收益和全部的水池负担是比LYPJ的那些显著地小的,并且越大,越多并且越多降低种子背景率负担更差的谷物充满。交通率每YLY6的区域韧皮部比LYPJ或SY63的大。Theresults建议YLY6拥有强壮的来源,大水池活动和有效流动,它为它充满的高种子背景率和好谷物放了一个生理的底。
简介:Rice(Oryzasativa)issensitivetosalinity,butthesalttoleranceleveldiffersamongcultivars,whichmightresultfromnaturalvariationsinthegenesthatareresponsibleforsalttolerance.High-affinitypotassiumtransporter(HKTs)hasbeenproventobeinvolvedinsalttoleranceinplants.Therefore,wescreenedfornaturalnucleotidepolymorphisminthecodingsequenceofOsHKT1,whichencodestheHKTproteinineightVietnamesericecultivarsdifferinginsalttolerancelevel.Intotal,sevennucleotidesubstitutionsincodingsequenceofOsHKT1werefound,includingtwonon-synonymousandfivesynonymoussubstitutions.Furtheranalysisrevealedthatthesetwonon-synonymousnucleotidesubstitutions(G50TandT1209A)causedchangesinaminoacids(Gly17ValandAsp403Glu)atsignalpeptideandtheloopofthesixthtransmembranedomain,respectively.Toassessthepotentialeffectofthesesubstitutionsontheproteinfunction,the3DstructureofHKTproteinvariantswasmodelledbyusingPHYRE2webserver.Theresultsshowedthatnodifferencewasobservedwhencomparedthosepredicted3DstructureofHKTproteinvariantswitheachother.Inaddition,thecodonbiasofsynonymoussubstitutionscannotclearlyshowcorrelationwithsalttolerancelevel.Itmightbeinterestingtofurtherinvestigatethefunctionalrolesofdetectednon-synonymoussubstitutionsasitmightcorrelatetosalttoleranceinrice.
简介:ThecompleteopenreadingframeofOsPIN1awasamplifiedthroughreversetranscriptase-polymerasechainreaction(RT-PCR)basedonthesequencedepositedinGenBanktoexploretherelationshipbetweentheauxineffluxproteinOsPIN1aandthenegativephototropismofriceroots.SequencingresultsshowedthattheGCcontentofOsPIN1awas65.49%.ThefusionexpressionvectorpCAMBIA-1301-OsPIN1a::GFPcontainingtheOsPIN1ageneandacodinggreenfluorescentprotein(gfp)genewasconstructed.ThefusionvectorwastransferredintoonionepidermalcellsbyAgrobacteriumtumefacienstransformation.ThetransientexpressionofOsPIN1a-GFPwasmainlylocatedinthenucleusandcellmembrane.Moreover,thetransgenicplantswereobtainedbyAgrobacterium-mediatedgenetictransformation.MoleculardetectionperformedbyusingPCRandβ-glucuronidasestainingshowedthatthetargetconstructwasintegratedintothegenomeofrice.Thenegativephototropiccurvaturesofthetransgenicricerootswerehigherthanthoseofthewildtype.Similarly,theexpressionlevelsofOsPIN1ainthetransgenicplantswereconsiderablyhigherthanthoseinthewild-typeplants.TheseresultssuggestthatOsPIN1aiscrucialinthenegativephototropiccurvatureofriceroots.
简介:Membersoftheactivityofbc1complex(ABC1)familyareproteinkinasesthatarewidelyfoundinprokaryotesandeukaryotes.PreviousstudiesshowedthatseveralplantABC1genesparticipatedintheabioticstressresponse.Here,wepresentthesystematicidentificationofriceandArabidopsisABC1genesandtheexpressionanalysisofriceABC1genes.Atotalof15and17ABC1genesfromthericeandArabidopsisgenomes,respectively,wereidentifiedusingabioinformaticsapproach.Phylogeneticanalyseso...
简介:米饭镉(Cd)敏感变异的cadB-1用Agrobacteriumtumefaciens被获得调停的系统。在cadB-1和野类型(WT)的暴露以后米饭幼苗到为有增加外部Cd集中的cadB-1和WT的10d,在根积累到高水平的Cd,茎和叶子的Cd集中的一个范围,并且在cadB-1的幼苗生长的抑制比在WT更严肃。氢过氧化物累积在cadB-1的叶子和根是更高的。减少的谷胱甘肽(GSH)的比率/oxidized谷胱甘肽(GSSG),ascorbate(ASC)/dehydroascorbate(DHA)和减少的菸碱腺嘌dinucleotide磷酸盐(NADPH)/oxidized菸碱腺嘌dinucleotide磷酸盐(NADP+)在高Cd层次下面在叶子和根两个都比在WT在cadB-1是更低的。ascorbateperoxidase(APX)的活动,谷胱甘肽peroxidase(GR),dehydroascorbatereductase(DHAR)和monodehydroascorbatereductase(MDHAR)在Cd的高水平的处理下面在叶子和根两个都比在WT在cadB-1是也更低的。我们的结果建议在Cd应力下面,ASC-GSH周期更严重比在WT在cadB-1被禁止,显示变异的cadB-1不太能清除反应的氧种类并且对Cd敏感。
简介:一个实验被进行用荧光灯的微分显示器(软式磁碟机)方法在干旱应激和正常条件下面在米饭叶子和根比较信使rna表达式差别。一积极碎片被H.A的联合孤立黄页(contained0.1%H.A。黄)分离和宏数组屏蔽方法。比作ArabidopsisthalianaNADPH氧化还原酶基因,它有96%身份。cDNA是1423bp,并且包含了与345氨基酸残余编码蛋白质的1048bp的一个完全的开的读物框架。而且,基因表示水平在正常条件下面比那在干旱应激下面是更高的。在干旱反应下面的NADPH氧化还原酶基因的可能的角色也被讨论。
简介:到在米饭germplasm91-1A2的米饭胆量小蚊的抵抗被识别并且遗传上分析了。米饭人口的F1s从作为一个男父母与米饭材料Jinggui,TN1,W1263(Gm1),IET2911(Gm2),BG404-1(gm3),OB677(Gm4),ARC5984(Gm5)和Duokang1(Gm6)交叉的91-1A2被导出。到米饭胆量小蚊的所有父母线和F1,BC1F1和F2人口的抵抗被识别。结果证明91-1A2和所有F1s对中国米饭胆量小蚊遗传因子型IV抵抗。到在BC1F1和F2的易受影响的抵抗植物的分离比率被X2测试与1:3和9:7规则给予,建议到中国米饭胆量小蚊遗传因子型IV的91-1A2的抵抗被是新抵抗基因的二主导的基因控制,对已知的米饭胆量小蚊抵抗基因非突变而产生之遗传因子。