简介:AsoilpotcultureexperimentwithfoursuppliedPlevels(i.e.P30,P50,P100,P200,representingsupplementalP30,50,100,200mg/kg,respectively)wasconductedtoinvestigateuptakeanduseabilitytoPandZninthericegenotypeswithdifferentP-efficiency,ofwhichricegenotypes508,99011,580,99112werelow-Ptolerantand99056,99012werelow-Psensitive.Low-Ptolerantrice580and99011absorbedmorePthantheothers,andricegenotype580hadstrongeruptakeabilityespeciallyatlow-PlevelsuchasP50andP30.508couldabsorbconsiderableP,andhadthelowestPpercentageofshoot,indicatingithadgoodperformanceinP-useefficiency.ThesethreericegenotypeshadlargerbiomassandlessresponsetochangedPlevelthanricegenotype99112,99056and99012.Ricegenotype99112showedLow-Ptolerancemainlybysacrificingbiomasstomaintainhighrelativegrainyield.TheleastamountofPabsorbedby99056showedithadthelowestPuptakeefficiency,andthehighestPpercentageinshootof99012meantithadthelowestPuseefficiency.Sotheytwoshowedlow-Psensitivity.ZncontentsinshootunderP200,P100andP50weresimilar,butP30increasedZncontentinshootsignificantly.TheZncontentsinshootof99112,99056and99012werehigherthanthoseof508,99011and580,especiallyattilleringstageandbootingstage.AsfortotalZncontentinshoot,Low-Ptolerantricegenotype580hadthelargestamountandfollowedby99011and508,low-Ptolerantricegenotype99012hadthesmallestamountatthethreesamplingstageandfollowedby99056.Furthermore,P/Zninshootof99012wasthehighest,andthatof99056wasthesmallestatthesamePlevel.
简介:ThehistoricaltransitionoftimberdistributionpatterninChinawaspresentedfirstlywithmainpartsoftimberflowsinChina,whichincludedtimbermarkets,timbertransportsystemandregulationsoftimbertransport.BasedontheoverviewontropicaltimberflowsinChina,thetradeflowsoftropicaltimberfromproductionandimportsoftropicaltimbertoexportoftropicaltimberproductswereanalyzedbyvividillustrations.Atthesametime,problemsintimbertradeflowsinChinawereanalysedintheend.
简介:本文综述了在杀虫剂抗性中起重要作用的P450酶系研究的最新进展,内容包括:细胞色素P450酶系基因及其基因的表达与调控,P450介导抗性的分子基础。细胞色素P450表达表现出发育期,组织,品系特异性及可诱导性。P450表达的调控机制复杂,可能受顺式调控元件(如CYP6B1)或反式作用因子(如CYP6A1)或顺式,反式因子的共同调控(如CYP6D1),调控可能涉及转录增强的转录机制或mRNA稳定性增加的转录后机制。P450的超量表达是P450酶系介导抗性的主要机制,P450的氨基酸替换也可能在杀虫剂抗性中起作用。
简介:AdsorptionofCr(VI)andp-chloroanilineonthreetypicalsoilcolloidsandpHinfluencewerestudiedusingbatchequilibriummethod.BothofCr(VI)andp-chloroanilineadsorptiononthecolloidscouldbewelldescribedbygeneraladsorptionsimulationequations,TheadsorptionprocesseschangedwithmediapH.WhenCr(VI)andp-chloroanilinecoexistedonsoilcolloids,theirinteractionscouldbeobservedinacertainpHrangetobeaccompaniedwithCr(VI)reduction,whichclearlysuggestedthatasurfacecatalyticreactionoccurredinthissystem.SoilcolloidactedasaneffcientcatalystfortheinteractionofCr(VI)andp-chloroaniline.ThepHvaluesatwhichnointeractionwasobservedwere4.0,4.5and5.0forthecolloidsofindigoticblacksoil,yellow-brownsoilandlatosol,respectively,Capillaryelectrophoresisusedtoanalyzep-chloroanilineprovidedahighseparationefficiencyandshortseparationtime,andneedednomoreextensivepretreatmentofsamples.
简介:以抑菌活性生物测定结果为指导,采用硫酸铵沉淀、离子交换柱层析、凝胶柱层析和SDS一聚丙烯酰胺凝胶电泳等方法,从采自广西土壤的1株放线茵菌株GXWl发酵液中分离到1种未见文献报道具有抑菌活性的蛋白,经质谱鉴定并与NcBInr蛋白数据库比对,认为其应为磷酸盐结合蛋白前体(phosphate—bindingproteinprecursor,pre—PBP),分子质量为39.18ku。根据其形态特征、培养特征、生理生化特性及16SrDNA序列分析,将菌株GXWl归入链霉菌属,并鉴定为锈赤蜡黄链霉菌Streptomycesrubiginosohelvolus。
简介:共进行两项试验,第一项按丹宁分析法F-D法(Folin-Dennismethod)的丹宁提取程序提取高粱中的丹宁后又用PEG(聚乙二醇,M=4000)对高粱进行处理,然后测定处理前后高粱蛋白质的胃蛋白酶消化率,第二项试验分别按含丹宁饲料;大豆粕的不同处理比,用高粱,青杠叶,刺槐叶的浸提液及化学纯丹宁酸溶液处理大豆粕,然后测定豆粕蛋白质的胃蛋白酶消化率,结果表明,未提取丹宁的高粱蛋白质消化率仅为14.19%。提取丹宁后提高到18.24%,而PEG处理后提高了90%,达27.03%。大豆粕蛋白质胃蛋白酶消化率因浸提液处理,消化率由72.68%下降至20.88%,三者中又以刺槐叶浸提液对消化率影响最强,高粱浸提液最弱,而丹宁酸在处理比达6%,大豆蛋白质消化率达60%左右时,再增加丹宁酸处理比,消化率不再继续