简介:Synchronousgammaoscillationsarebelievedtoplayaprominentroleintheinformationprocessingofbiologicalneuralsystems.Experimentalobservationshavefoundthatthefrequencyandpowerofgammaoscillationsintheprimaryvisualcortex(V1zone)areregulatedbytheilluminationcontrastofvisualstimulus.However,theunderlyingmechanismofhowthesynchronousoscillationsdependontheilluminationcontrasthasnotbeenwellexplained.Weproposealocalexcitatory/inhibitory(E/I)neuronalnetworkofintegrate-and-fire(IAF)neuronswiththedifference-of-Gaussians(DOG)receptivefieldtounveilthismechanism.Simulationresultsdemonstratethatthehighertheilluminationcontrast,thehigherthefrequencyofgammaoscillations.Thepowerofgammaoscillationsalsoincreaseswiththeincreaseofilluminationcontrast.Theseresultsareconsistentwiththeexperimentalfindings.
简介:摘要目的探讨靶向B细胞淋巴瘤的分子探针131I-ch4E5的制备方法及其对荷人B细胞淋巴瘤裸鼠的抑瘤作用。方法采用Iodogen法用131I标记抗CD80的人-鼠嵌合抗体ch4E5,采用放射性薄层色谱扫描法测定标记率和放射化学纯度。(1)体外实验:在含细胞数为1×108、1×109、1×1010、5×1010、1×1011个/L的人B细胞淋巴瘤Raji细胞的离心管中分别加入131I-ch4E5溶液,同时设置非特异结合对照管,测量每管沉淀的放射性计数,计算Raji细胞与131I-ch4E5的特异性结合率。(2)体内实验:3只荷瘤裸鼠尾静脉注射7.4 MBq的131I-ch4E5,72 h后处死,分别取肿瘤、血等14种脏器或组织,分别计算肿瘤与正常组织的放射性比值(T/NT);将15只荷瘤裸鼠按随机数字表法分为3组,分别经尾静脉注射131I-ch4E5(131I-ch4E5组)、未标记的ch4E5(ch4E5组)及生理盐水(对照组),观察3组荷瘤裸鼠的肿瘤生长情况,给药后第15天计算肿瘤生长抑制率;之后处死荷瘤裸鼠取肿瘤组织,采用苏木精-伊红染色法做组织病理学检查。2组间的比较采用两独立样本t检验。结果131I-ch4E5的标记率为(84.2±2.4)%、放射化学纯度为(97.1±1.1)%。(1)体外实验:131I-ch4E5与Raji细胞的结合率随细胞数量的增加而升高,最大结合率为(38.2± 2.3)%。(2)体内实验:131I-ch4E5在荷人B细胞淋巴瘤裸鼠体内的分布具有靶向性,肿瘤与肌肉的T/NT最大为7.30;与ch4E5组、对照组比较,131I-ch4E5组肿瘤生长减慢,差异均有统计学意义(t=2.889、6.516,均P<0.05);给药后第15天,131I-ch4E5组肿瘤抑制率为71.7%、ch4E5组为43.4%。组织病理学检查结果同样显示131I-ch4E5的治疗效果更明显。结论自制分子探针131I-ch4E5的标记率及放射化学纯度高,且对荷人B细胞淋巴瘤裸鼠肿瘤生长有明显的抑制作用,为进一步研究131I-ch4E5在放射免疫治疗中的应用提供了实验依据。
简介:InviewofthelimitationsofaRn-GnmodelinthelowfrequencyrangeandthedefectsofanEn-Inmodelincommonusenow,thispaperbuildsacompleteEn-Inmodelaccordingtothetheoryofrandomharmonic.Theparametersforthelow-noisedesignsuchastheequivalentinputnoisyvoltageEns,theoptimumsourceimpedanceZsoptandtheminimumnoisefigureFmincanbecalculatedaccuratelybyusingthisEn-Inmodelbecauseitconsidersthecoherencebetweenthenoisesourcesfully.Moreover,thispaperpointsoutthatitwillcausethemaximum30%miscalculationwhenneglectingtheeffectsofthecorrelationcoefficient7.Usingtheseries-seriescircuitsasanexample,thispaperdiscussesthemethodsfortheEn-InnoiseanalysisofelectroniccircuitspreliminarilyanddemonstratesitscorrectnessthroughthecomparisonbetweenthesimulatedandmeasuredresultsoftheminimumnoisefigureFminofasinglecurrentseriesnegativefeedbackcircuit.