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简介：Wehavereviewedthegenetherapyingastrointestinaldiseases^[1].GastriccanceriscommoninChina^(2-20),anditsearlydiagnosisandtreatmentarestilldifficultuptonow^(13-36).Theex-pressionofanexogenousgeneintroducedbygenetherapyintopa-tientswithgliomascanbemonitorednon-invasivelybypositron-emissiontornography^[4].

简介：AbstractGastric cancer (GC) is a common malignancy and is the third leading cause of cancer-related death. At present, there is no simple and effective screening method for early-stage GC, and the treatment results and prognosis are poor. With the continuous improvement of molecular biology techniques, research on circular RNA (circRNA) has gradually expanded over time. Much data supports the role of circRNA in tumorigenesis. Moreover, due to its structural specificity and biological stability, circRNA is anticipated to be a potential biomarker for tumor diagnosis. Studies have confirmed that circRNA can participate in the proliferation, invasion, metastasis, and apoptosis of GC. These findings will lead to novel directions for the diagnosis and treatment of GC. This article reviews the structure and function of circRNA, summarizes the current studies on circRNA, and discusses the potential diagnostic value of circRNA in GC.

简介：OurpreviousstudyshowedthattwoMKN45variants,namelyMKN45Lm-andMKN45Lm+selectedbylamininadhesioninvitro,haddifferentabilitiesofinvasionandmetastasisinvivo(inoculatedinnudemice)andinvitro(inBoydenchamber),alsohaddifferentexpressionofcysteineproteinase.Inthepresentstudy,weinvestigatedthecellapoptosisofthisMKN45variantsbyTUNELandFACSmethods.Theresultswereshownsignificantdifferentofcellapoptosisinthiscellsublines.TheMKN45Lm-cellshadasmallsubdiploidypeak(3.5%)inDNAcontentanalyzedbyflowcytometry,

简介：Earlydiagnosisandtreatmentisthekeytoimprovingtheprognosisofgastriccancer.Thepastdecadeshavewitnessedtherapidadvancesinthediagnosisandmanagementofearlygastriccancer(EGC):endoscopyhasplayedanincreasinglyimportantrole,whereaslaparoscopictechniqueshavealsobeenintroducedforEGCtreatment.InChina,the

简介：Esophagealandgastriccancer(GC)arerelatedtoobesityandbariatricsurgery.Riskfactors,suchasgastroesophagealrefluxandHelicobacterpylori,mustbeinvestigatedandtreatedinobesepopulation.Aftersurgery,GCreportsareanecdotalandtreatmentisnotstandardized.ThisreviewaimstodiscussGCrelatedtoobesitybeforeandafterbariatricsurgery.

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简介：AIM:Tounderstandthecorrelationofserumcholinesterase(CHE)activitywithgastriccancerandtoassesstheirclinicalsignificance.METHODS:ThevelocitymethodwasadoptedtodetecttheactivityofserumCHEinpatientswithgastriccancerandinpatientswithnon-malignanttumorascontrols.RESULTS:TheserumCHEactivityinthetreatmentgroupwassignificantlylowerthanthatinthecontrolgroupwithaverysignificantdifferencebetweenthetwogroups(83.3:113.1,P=0.0003).Agewassignificantlyassociatedwiththeincidenceofgastriccaner.CONCLUSION:SerumCHEactivityhasacloserelationwiththeincidenceofgastriccancer.

简介：SpleencellsfromBalb/cmiceimmunizedwithfivehumangastriccancercelllinesinsequencewerefusedwithmurinemyelomacelllineSP2/0,andhybridomas3F4,3G9and3H11,secretingmonoclonalantibodiees(MoAbs)againstgastriccancer,wereobtainedthroughselectivecultureandscreening.TheseMoAbshavebothgoodselectivityandahighpositiverateofreactionforgastriccancer,reaching5/5and84.8%to93.5%forgastriccancercellsandtissuesrespectively.ThereactionofMoAbswithnormalcellsandtissueswasneglible.ThecorrespondingantigensoftheMoAbsweresensitivetodigestionbytrypsinandpronaseandresistanttotreatmentwithsodiumperiodate,indicatingtheirnatureasproteins.Theantigen3G9couldbevisualizedwithWesternblottingastwobandswithmolecularweightsof100KDand70KD,howevernobandwasfoundforantigens3F4and3H11.Therewasahighexpressionofantigensinthemajorityofgastriccancercellsandtissuesindependentofhis-topathologicaltypeof

简介：瞄准：为了孤立并且识别差别，由二维的电气泳动(2-DE)和帮助矩阵的激光解吸附作用/电离time-of-flight团分光术(MALDI-TOF-MS)表示了在癌症和胃的癌症的正常纸巾之间的蛋白质。方法：胃的癌症纸巾和配对的正常纸巾的可溶的部分蛋白质被2-DE分开。差别表示了蛋白质被MALDI-TOF-MS和数据库搜索选择并且识别。结果：有高分辨率和重制度的2-DE侧面被获得。23个蛋白质点从染色胶化的裂片被切除并且由胰岛素，十五个蛋白质点成功地在被识别在胶化消化了。在识别蛋白质之中，有十过去表示并且在胃癌症纸巾的五下面表示的蛋白质与正常纸巾相比。结论：在这研究，人的胃的癌症织物和配对的正常织物的解决得好的、可再现的2-DE模式被建立并且优化并且某些表示差别的蛋白质被识别。2-DE和MS的联合使用提供一条有效途径为潜在的肿瘤标记屏蔽。

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简介：AbstractGastric cancer (GC) is one of the most common malignant tumors worldwide. Its incidence ranks the 5th among all malignant tumors globally, and it is the 3rd leading cause of death among patients with cancer. Surgical treatment is the first choice in clinical practice. However, targeted therapy, immunotherapy, and other treatment methods have also become research hotspots at home and abroad with the development of individualized precision therapy in recent years, besides traditional radiotherapy and chemotherapy. At present, targeted therapy and immunotherapy are methods used for treating GC, and they have important clinical application value and prospects. This study aimed to review the research progress of targeted therapy and immunotherapy for GC, focusing on its mechanism of action and related important clinical trials, hoping to provide references for the clinical treatment of GC.

简介：Objective:Theexpressionoftumorbiomarkersmaychangeafterchemotherapy.However,whethersecretedproteinacidicandrichincysteine(SPARC)expressionchangesafterchemotherapyingastriccancer(GC)isunclear.ThisstudyinvestigatedtheinfluenceofchemotherapyonSPARCexpressioninGC.Methods:ImmunohistochemistrywasusedtoanalyzeSPARCexpressionin132GCcases(including54caseswithpreoperativechemotherapyand78caseswithoutpreoperativechemotherapy).SPARCexpressionofpostoperativespecimenswithandwithoutpreoperativechemotherapywasassessedtoanalyzetheinfluenceofchemotherapyonSPARCexpression.Results:SPARCwashighlyexpressedinGCcomparedwiththedesmoplasticstromasurroundingtumorcellsandnoncanceroustissues.HighSPARCexpressionwascorrelatedwithinvasiondepth,lymphnode,andTNMstage.Afterchemotherapy,alowerproportionofhighSPARCexpressionwasobservedinpatientswithpreoperativechemotherapythaninthecontrols.For54patientswithpreoperativechemotherapy,grosstype,histology,depthofinvasion,lymphnode,TNMstage,andSPARCexpressionwererelatedtooverallsurvival.Furthermultivariateanalysisshowedthatlymphnode,histology,andSPARCexpressionafterchemotherapywereindependentprognosticfactors.Conclusion:SPARCexpressionmaychangeafterchemotherapyinGC.SPARCexpressionshouldbereassessedforpatientswithGCafterchemotherapy.

简介：InthedevelopedWesternpartoftheworld,theincidenceofGCAhasmarkedlydecreasedoverthepastdecades.ThisdecreaseoftheGCAincidenceseemstobeageneralandglobalevent,suggestingthatoneorsomegloballyandgenerallycommonfactorsplayacriticalroleinthepathogenesisofGCA,andthatthisfactororfactorshavedecreasedininfluenceworldwidelyduringthepastdecades.Theseetiopathogeneticfactorshardlyareexoticdifferencesinlocalhabitsofeatingordrinkingonly.SuchexoticandlocalfactorscannotexplainthestrikingconsistencythatisapparentintheglobalepidemiologyofGCA.RegardingtheH.pyloriinfectionasthekeyfactorinthepathogenesisofgastriccancer,theserequirementsofglobalityarefulfilled.

简介：AbstractBackground:Texture analysis (TA) can quantify intra-tumor heterogeneity using standard medical images. The present study aimed to assess the application of positron emission tomography (PET) TA in the differential diagnosis of gastric cancer and gastric lymphoma.Methods:The pre-treatment PET images of 79 patients (45 gastric cancer, 34 gastric lymphoma) between January 2013 and February 2018 were retrospectively reviewed. Standard uptake values (SUVs), first-order texture features, and second-order texture features of the grey-level co-occurrence matrix (GLCM) were analyzed. The differences in features among different groups were analyzed by the two-way Mann-Whitney test, and receiver operating characteristic (ROC) analysis was used to estimate the diagnostic efficacy.Results:InertiaGLCM was significantly lower in gastric cancer than that in gastric lymphoma (4975.61 vs. 11,425.30, z = -3.238, P = 0.001), and it was found to be the most discriminating texture feature in differentiating gastric lymphoma and gastric cancer. The area under the curve (AUC) of inertiaGLCM was higher than the AUCs of SUVmax and SUVmean (0.714 vs. 0.649 and 0.666, respectively). SUVmax and SUVmean were significantly lower in low-grade gastric lymphoma than those in high grade gastric lymphoma (3.30 vs. 11.80, 2.40 vs. 7.50, z = -2.792 and -3.007, P = 0.005 and 0.003, respectively). SUVs and first-order greylevel intensity features were not significantly different between low-grade gastric lymphoma and gastric cancer. EntropyGLCM12 was significantly lower in low-grade gastric lymphoma than that in gastric cancer (6.95 vs. 9.14, z = -2.542, P = 0.011) and had an AUC of 0.770 in the ROC analysis of differentiating low-grade gastric lymphoma and gastric cancer.Conclusions:InertiaGLCM and entropyGLCM were the most discriminating features in differentiating gastric lymphoma from gastric cancer and low-grade gastric lymphoma from gastric cancer, respectively. PET TA can improve the differential diagnosis of gastric neoplasms, especially in tumors with similar degrees of fluorodeoxyglucose uptake.

简介：AbstractGastric cancer (GC) is one of the most common malignant tumors. The mechanism of how GC develops is vague, and therapies are inefficient. The function of microRNAs (miRNAs) in tumorigenesis has attracted the attention from many scientists. During the development of GC, miRNAs function in the regulation of different phenotypes, such as proliferation, apoptosis, invasion and metastasis, drug sensitivity and resistance, and stem-cell-like properties. MiRNAs were evaluated for use in diagnostic and prognostic predictions and exhibited considerable accuracy. Although many problems exist for the application of therapy, current studies showed the antitumor effects of miRNAs. This paper reviews recent advances in miRNA mechanisms in the development of GC and the potential use of miRNAs in the diagnosis and treatment of GC.

简介：TheallelicdistributionofEcoRIandBamHIfragmentsofrasfamilygenesbetweenthehumanprimarygastriccancertissuesandthecorrespondingadjacentnormaltissuesdidnotshowanydifferences.ThreegenotypesofBamHIrestrictionfragmentslengthpolymorphismofc-H-raswererevealed.NosignificantdifferencesintheRFLPswereobservedbetweennormalindividualsandgastriccancerpatients.Fourprotooncogenes,c-H-ras,N-ras,c-mycandc-fos,werefoundtobetranscriptionallyactiveinthegastriccancertissuesinsomecasesexamined.Thecomparisonoftheexpressionoftheseoncogenesbetweenthemalignanttissuesandthecorrespondingnormaltissuesshoweddifferentialpatterns.Theexpressionofc-H-rasatcellularlevelwasdetectedwithinsituhybridization.Theenhancedexpressionofc-H-rasinthegastriccancercellswasdemonstrated,butthedegreeoftheexpessionamongthecancercellswasshowntobeheterogeneous.Inaddition,theenhancedexpressionofc-H-raswasseeninthei

简介：客观：在主要胃的癌症的开始和发展调查染色体错误和他们的角色。方法：从稳固的肿瘤的改进、直接染色体准备为分析在decolored乐队G染色体上由鱼跟随了的乐队G被采用以便染色体错误能在DNA水平被证实。结果：28个主要胃的癌症标本的一个总数是研究。大小写1和大小写2有的简单染色体数字变化：49，XY，+2，+8，+9并且48，+8，+20分别地。几乎，大小写1和2有复杂染色体畸形。经常的出现结构的染色体深奥del(7q)(21/26)，del(3p)(14/26)，del(lp)(U/26)和del(17p)(10/26)。染色体畸形能简单、复杂。在前者，包含1～3个染色体的数字变化能被观察。8和9可能代表的三染色体性主要胃的癌症的细胞发生的亚群。在里面以后，del(7q)是最一致的错误。7q32-qter是通常失去的片断结论：染色体的数字、结构的改变在主要胃的癌症是在场的。Del(7q)是主要胃的癌症的结构的变化特征之一。在7q32-qter碎片，肿瘤suppressor基因可能存在并且它可以有靠近的关系到胃的癌症的开始和前进。

简介：Baseduponstudiesfromrandomizedclinicaltrials,theextended(D2)lymphnodedissectionisnowrecommendedasastandardprocedureforlocaladvancedgastriccancerworldwide.However,therationalextentlymphadenectomyforlocaladvancedgastriccancerhasremainedatopicofdebateinthepastdecades.Duetothelimitationoflowmetastaticrateinpara-aorticnodes(PAN)inJCOG9501,theclinicalbenefitofD2+para-aorticnodaldissection(PAND)forpatientswithstageT4and/orstageN3disease,whichisverycommoninChinaandothercountriesexceptJapanandKorea,cannotbedetermined.Furthermore,theroleofsplenectomyforcompleteresectionofNo.10andNo.11nodeshasbeencontroversial,andhowever,thefinalresultsfromtherandomizedtrialofJCOG0110haveyettobecompleted.GastriccancerwiththeNo.14andNo.13lymphnodemetastasisisdefinedasM1stageinthecurrentversionoftheJapaneseclassification.WeproposethatD2+No.14vand+No.13lymphadenectomymaybeanoptioninapotentiallycurativegastrectomyfortumorswithapparentmetastasistotheNo.6nodesorinfiltratetoduodenum.Theexaminedlymphnodeandextranodalmetastasisaresignificantlyassociatedwiththesurvivalofgastriccancerpatients.