简介：AbstractBackground:Patients carrying the HongKongαα (HKαα) allele and -α3.7/αααanti-4.2 could be misdiagnosed as -α3.7/αα by the current conventional thalassemia detection methods, leading to inaccurate genetic counseling and an incorrect prenatal diagnosis. This study was aimed to accurately analyze the genotypes of HKαα carriers and -α3.7/αααanti-4.2.Methods:Samples were collected in our hospital from July 2017 to October 2019. Twenty-four common types of Chinese thalassemia were screened by gap-polymerase chain reaction (Gap-PCR) and reverse dot blot (RDB). Anti-4.2 multiplex-PCR was used to confirm carriers of the αααanti-4.2 duplication with -α3.7 deletion. Two-round nested PCR and multiplex ligation-dependent probe amplification (MLPA) were applied to accurately identify and confirm their genotypes. For data analysis, we used descriptive statistics and Fisher’s exact tests.Results:Two thousand five hundred and forty-four cases were identified as thalassemia in 5488 peripheral blood samples. The results showed that α, β, and αβ compound thalassemia were identified in 1190 (46.78%), 1286 (50.55%), and 68 (2.67%) cases, respectively. A total of 227 samples from thalassemia patients were identified as -α3.7/αα by Gap-PCR, and the genotypes of two samples were uncertain. There was a difference between Gap-PCR and combined groups (Gap-PCR combined with nested PCR and MLPA) in detecting HKαα (P < 0.05). Among the 229 patients, 20 patients were identified as HKαα carriers and one was identified as -α3.7/αααanti-4.2 by two-round nested PCR and MLPA, including 15 patients with HKαα/αα, three with HKαα/αα and β-thalassemia coinheritance, one with HKαα/-SEA, one with HKαα/-α4.2 and β-thalassemia coinheritance, and one with -α3.7/αααanti-4.2 and β-thalassemia coinheritance.Conclusions:αααanti-4.2 and HKαα genotypes of patients carrying -α3.7 need to be detected to reduce the misdiagnosis rate of patients carrying HKαα and -α3.7/αααanti-4.2 alleles. More accurate genetic counseling can be provided in the clinic using nested PCR combined with MLPA.

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简介：RapiddifferentialidentificationofMycobacteriumspeciesisessentialforeffectivediagnosisandmanagementofmycobacteriosis.Theaimofthisstudywastodevelopanovelmultiplexprobearraybasedonthe16S-23SrRNAgeneinternaltranscribedspacersequenceforthegenotypingofmycobacteriatothespecieslevel.Apairofprimersandasetofgenus-andspecies-specificprobesweredesignedfromtheconservedandpolymorphicregionsofthe16SrRNAgene,internaltranscribedspacer,and23SrRNAgenesequencesofmycobacteria.Weusedanovelmultiplexprobearrayforidentificationof266clinicalspecimensobtainedfrompatientswithmycobaterialinfection.Theresultsshowedthattheoverallspecificityandsensitivityofournovelprobearraywereboth100%forthegenus-specificprobeandMycobacteriumtuberculosiscomplex-specificprobe.Therewere79.3%(23/29)ofnontuberculousmycobacteriawhichcouldbeidentifiedtothespeciesleveldirectlyinthespecimensfromChina.SomeintraspeciesheterogeneityinM.avium,M.intracellulare,M.chelonaeandM.abscessuswasobserved.Withtheincreaseofsequencesofinternaltranscribedspacerandnumbersofwholemicrobialgenomes,andfurtheroptimizationofprobes,themultiplexprobearraywillbecomeapromisingtoolfortherapidandaccurateidentificationofmycobacteriainordinaryclinicallaboratories.

简介：中子质子比率的动能光谱上的动量依赖者相互作用的效果[?](n/p)气体[?]为64Zn+64Zn的b(Ek)被学习。它被发现那[?](n/p)气体[?]b(Ek)易感知地取决于动量依赖者相互作用并且微弱地在在里面中等的核子核子上穿过节和对称潜力。因此[?](n/p)气体[?]b(Ek)是为在重离子碰撞在动量依赖者相互作用上提取信息的一根可能的探针。[从作者抽象]

简介：全身的豺狼座erythematosus(SLE)的一个特点是由汽车反应的B房间的各种各样的自身抗体的一致生产。Interferon-α；(IFN-α；)发信号高度在SLEB房间被激活并且由B房间在抗体反应起一个重要作用。以前的研究证明了CD180否定的B房间，戏剧性地在SLE病人被增加，为自身抗体的生产负责。然而，在CD180和IFN-α之间的协会；未知的发信号的遗体。在现在的学习，我们在调整IFN-α的激活上探索了CD180的效果；在B房间发信号。我们发现CD180否定的B房间的数字在MRL/Mp-Fas(lpr/lpr)被增加豺狼座容易的老鼠与野类型的老鼠相比。Phenotypic分析证明CD180否定的B房间包括了CD138+plasmablast/plasma房间和GL-7+幼芽的中心(GC)B房间。尤其是，CD180的结扎显著地禁止了IFN-α；信号变换器的导致的phosphorylation和抄写2的使活跃之物(STAT-2)和以在vitro的一种Lyn-PI3K-BTK-dependent方式的刺激IFN的基因(ISG)的表示。而且，CD180的结扎能也禁止IFN-α；在在vivo的B房间的导致的ISG表示。而且，像使用费的受体7并且像使用费的受体9发信号小径能显著地downregulateCD180表示并且调制在IFN-α的激活上发信号的CD180的禁止的效果；发信号。一起，我们的结果加亮在CD180否定的B房间的增加的比例和IFN-α的激活之间的靠近的协会；在SLE发信号。我们的数据提供分子的卓见进IFN-α的机制；在为SLE处理的SLEB房间和一条潜在的治疗学的途径的发信号的激活。

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简介：Fungalpathogenscausingpoplarcankerdiseasesarecosmopolitaninspecies,andhaveawiderangeofhosts.Thesepathogenshavediverseanamorphsandtheirmorphologyoverlapswitheachother;Theirteleomorphsarehardlydiscoveredinnature.Furthermore,theidentificationofthesepathogensisusuallylimitedbythegeography,hostandtaxonomicknowledge.Therefore,aculture-independentmethodusedtoidentifydeterminepathogensisexpectedtobedevelopedforfielddiagnostics.Throughamplifying,sequencingandanalyzingmultiplexnucleicacidtemplatesandgeneticmarkedtargetsequences,amultiplexPCRtechniquehasbeenestablishedandusedtodirectlydetectvariousenvironmentalsamples.Inthisstudy,fourpathogenstrainsandenvironmentalsampleswereamplifiedusingfungaluniversalprimersITS1/ITS4andEF1-728F/EF1-986RbygeneralPCRandmultiplexPCR.Theampliconsweresequenced,andthenalignedinGenBank.TheresultshowedthatthemultiplexPCRwasabletosuccessfullyamplifythetargetgeneandidentifythepathogensfromenvironmentalsamplesintheconditionofanannealingtemperatureof55.6℃andprimersfinalconcentrationof0.2μmol·L-1.ThemultiplexPCRcouldamplifythetargetattheconcentrationlevelofapproximatelylngofgenomicDNA.ThismethodwouldprovideausefultoolfortheidentificationofcankerpathogensbythemultiplexPCRandthehigh-throughputDNAmicroarraydetectionofenvironmentalsamples.

简介：Probesaretheinterfacebetweenmicrosystemsandbio-cells.Theidealinterfaceisone-to-oneinterface.Thoughvariousresearchgroupshavebeenabletoestablishsomesortofinterfacesaftermanyyearsofresearch,theyareverycrude.Neuronsaremillionsinnumbers,whereastheprosthesessuccessfullybuiltsofarhaveonlyafewhundredprobesatbest.Creatinganef-fectiveinterfaceisstillfaraway.Thoughwehavemicro-andnano-technologies,wecouldn’tbuildaprosthesiswithaneffectiveresolution.Mainreasonsbehinditarethetypeofprobebeingusedandthepoordesignoftheprobe.Toaddressthisproblem,wedevelopedamethodologytodesignaprobeandanarrayofprobeswithbetterresolutionandlessresistivedonutprobe.Thismethodologyhelpsustodesignaprobeoptimizingalltheparameters.Wepresentedourmethodologythroughadesignthatiscapableof70μmpenetrationinsidethetissue.Thetissueheatingbyourdesignedprobeisonly0.411°C.Wealsocharacterizedthedonutprobe,whichcouldbeusedbyanyresearchgrouptodesignadonutprobeoftheirspecificneed.

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简介：复合微分电压比较仪(MDVC)的一个新奇图案为减少电流和电源驱散被介绍。根据关系操作和逻辑操作的特殊性质，比较仪的部分在一些例子是冗余的，并且能因此被关掉。由选择并且切换当前的线路，几有效的微分对被单个尾巴电流stage-by-stage偏导，冗余的比较仪被切他们的尾巴电流关掉。作为结果，静止电流和电源消费极大地被减少。切换电流被输入完成微分对晶体管自己和因此没有额外的开关被要求。当MDVC很快被使用时analog-to-digital变换器(模数转换器)，它的当前的驱散比常规比较仪的低得多。这体系结构能也为逻辑操作在窗户比较仪，最大或最小的比较仪，和比较仪被使用。在所有这些盒子中的电源驱散能显著地被减少。

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简介：Anewsetupofmeasuringtemperatureisdeveloped,whichtheprobeisamicro-powerconsumptiveonewithCMOScircuitandisdrivenbyopticalpower.Fortransmittingthemeasuredsignalandopticalsignalinalongdistance,thefibertechnologyisappliedinthissetup.

简介：Vesiclecanbepreparedfromaqueousmixturesofsimplecommerciallyavailable,single-tailedcanonicandanionicsurfactants.Inthiswork,theI3/I1value,Ie/Imvalue,andfluorescencelifetimeofpyreneindifferentsystems(seethepreparationofsamples)weredetermined.Hieessentialaffectingfactorsintheformationofvesiclecanbededucedfromtheobtainedresults.Itshowedthatlargevesiclemustformnaturallybeforesonicationin0.082MoctyltrimethylammoniumbromideandsodiumlauratepH=9.2aqueoussolution.Whileaftersonication,onlysmallvesicleexists,whichcanbeprovedfurtherthroughelectronmicroscope.

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简介：Thepulseamplificationinthedispersion-decreasingfiber(DDF)isinvestigatedviasymboliccomputationtosolvethevariable-coefficienthigher-ordernonlinearSchr(o|¨)dingerequationwiththeeffectsofthird-orderdispersion,self-steepening,andstimulatedRamanscattering.Theanalyticone-solitonsolutionofthismodelisobtainedwithasetofparametricconditions.Basedonthissolution,thefundamentalsolitonisshowntobeamplifiedintheDDF.ThecomparisonoftheamplitudeofpulsesfordifferentdispersionprofilesoftheDDFisalsoperformedthroughthegraphicalanalysis.Theresultsofthispaperwouldbeofcertainvaluetothestudyofsignalamplificationandpulsecompression.

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