简介：AseismometerdataacquisitionunithasbeenusedintheChangpingseismicstationtorecordtheoutputofastrainmeter.Theoutputofastrainmeterwassampledatarateof100/secbyseismometeracquisitionfromtheoriginalrateof1perminute.Plentyofhighfrequencysampleddatawasrecorded.Theminutevaluecurvecalculatedfromtheseismometeracquisitionareconsistentwiththatoftheoriginaldatasampledbythestrainacquisitionsystem.Morecompletewaveformswererecordedwithahighersamplingrate,andseismicphaseparameterscalculatedbyusinghighersamplingratestrainseismicwavesarealsoinconsistencywiththeresultsofitspredecessors.SpectraofthestrainseismicwavesarecomparedwiththatofseismicwavesrecordedbyaseismometerintheShisanlingseismicstation,andtheirtrendsarealmostthesame.Besides,somelowerfrequencycomponentsstillexistinstrainseismicwaves.

简介：提炼效果(野类型和白类型水果上的1-(4-chlorophenyl)-3-(2,6-difluorobenzoyl)urea)杀虫药剂diflubenzuron飞果蝇melanogaster(Meigen，1830)被学习。成年昆虫在营养素被交给在小瓶杀虫药剂的不同集中(从diflubenzuron的0.048～48mg每营养素的1厘米3)。在每种情况中，昆虫显示出某死亡，它是集中家属。当比较两紧张时，我们能观察不同尖锐毒性，与对diflubenzuron更抵抗的野类型。然而，稍尖的毒性在两紧张以内是类似的。在两紧张，延长暴露导致了人口(>70%)的多数的扑灭。尽管意象能自由地交接，我们没观察到交接水果苍蝇，和我们没在任何暴露的组发现鸡蛋或幼虫。这些结果显示导致不同眼睛颜色的那个基因变化能也在暴露杀虫剂的区域影响昆虫的抵抗和幸存。

简介：Opticalfull-fieldmeasurementmethodsarenowwidelyappliedinvariousdomains.Ingeneral,thedisplacementfieldscanbedirectlyobtainedfromthemeasurement,howeverinmechanicalanalysisstrainfieldsarepreferred.Toextractstrainfieldsfromnoisydisplacementfieldsisalwaysachallengingtopic.Inthisstudy,afiniteelementmethodforsmoothingdisplacementfieldsandcalculatingstrainfieldsisproposed.Anexperimentaltestcaseonaholedaluminumspecimenundertensionisappliedtovali...

简介：TostudythegeneticcharacterizationoffourstrainsofBorreliaburgdorferiisolatedinChina.PCRtechniquewasusedtoamplifythe5S-23SrRNAintergenicspacerDNAfromthewholecellularDNAofisolatedGXLD-4,9,18andChang14,andthentheamplifiedproductswereclonedintoplasmidpGEM-TEasyandsequenced.Itwasfoundthatthe5S-23SrRNAintergenicspacerDNAofthefourisolateswas242bp,revealingthenucleotidesequenceidentityofmorethan99%.ThefourisolateshadhighersequenceidentifywithBorreliavalaisianathanwithothergeneticgroups.ThesefourisolatesmostlikelybelongtoBorreliavalaisianagenomicgroup.

简介：1089strainsofyeastswereobtainedfromseawater,seasediments,mudofseasalterns,gutsofmarinefishes,mangroveplantsandmarinealgae.Theresultsofroutineidentificationandmolecularanalysismethodsshowthat44strainsamongthemarineyeastsobtainedinthisstudybelongtoCandidatropicalis,whichmayindicateitswidedistributionindifferentenvironment,especiallyinthetropicalandsubtropicalmarineenvironment.ThewidedistributionofC.tropicalisindicatesthatitmayplayanimportantroleinmarineenvironmentandthemarineenvironmentinturnisagoodsourceforobtainingC.tropicalis.

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简介：Afundamentaltheoryfortheanalysisofresidualweldingstressesanddeformationbasedontheinherentstraindistributionalongtheweldedjointisintroduced.Distributionofinherentstrainsandlongitudinalresidualstressesinmediumthicknessplateweldmentiscalculatedandanalyzed.Anewmethodofcalculatinginherentstrainsandlongitudinalresidualstressesisproposed.

简介：Staphylococcusepidermidisisoneoftheleadingpathogensofnosocomialinfections.Twenty-eightstrainsofbiofilm-negativeStaphylococcusepidermidis,ofwhichthirteenstrainsconsideredastheclinicalstrainswereisolatedfromcatheters,bloodandurineofpatientsinHuashanHospital(Shang-hai,China)andfifteenstrainsconsideredascommensalstrainswereisolatedfromtheskinofhealthystudentswhohadnocontactwithhospitalsinrecenthalfyear,wereinvestigatedfortheadherencetohumanumbilicalveinendothelialcelllineECV304andhumancervicaladenocareinomacelllineHela,respectively.Ourresultsshowedthattheclinicalstrainsweremuchmoreadhesivetobothcellsthancommensalones.Theendothelialadherencemaybeavirulencefactorassociatedwiththisbacterialpathogenesis.

简介：能够lysing的所有以前报导的细菌的种类有害水藻从有害水藻花蕾在发生了的沿海的环境被孤立。由于在一个海藻的花蕾的水藻细胞溶解细菌的低集中，由存在方法孤立水藻细胞溶解细菌是困难的。在这篇论文，二水藻细胞溶解细菌的紧张，P01；P03，从在在移开水藻是高度有效的海绵上使不能调动的一个简历系统被孤立；microcystins。他们的lysing模式；Microcystisaeruginosa上的效果被学习了。降级为M这二紧张处理的结果表演。当时，与一阶的反应给予的aeruginosa当模特儿集中在从0～1000μgL的范围的叶绿素[1]。降级率常数是0.1067，0.1274；0.2792为P01；0.0683，0.0744；0.02897为P03当细菌的密度是8.6×1时0[5]，8.6×10[6]；8.6×10[7]房间mL[1]分别地。而且，二细菌的紧张在M上有赞成lysin的效果不仅。aeruginosa，而且上小球藻；Scene-desmus。他们M上的lysin的效果。aeruginosa没要求物理房间到房间接触，但是由一个细胞外的产品的生产继续。细菌的紧张被16SrRNA基因的PCR扩大作为杆菌种类识别，强风分析，；有在GenBank核苷酸数据库的序列的比较。

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简介：这研究被设计从A孤立endophytic真菌。mongholicus(在东北中国成长)决定他们是否能生产bioactive代谢物。endophytic真菌的四紧张(拉紧16，17，23和75)成功地从A被孤立。用表面消毒方法的mongholicus。根据ITS-rDNA序列分析，拉紧16和75作为镰刀霉oxysporum被识别，并且拉紧17和23作为Bionectriaochroleuca被识别。我们使用了Box-Behnken设计(BBD)优化液体发酵条件并且获得最大的房间干燥重量(CDW)产量。最佳的参数在下列试验性的条件下面被获得：第28i的温度口香糖的年龄。与那，新鲜口香糖增加了拍的成功。第二，本地人比较喜欢在温暖的季节期间拍，吗我们由决定Xac生长在30略潤潭慮?楤業畮慴?是最好的验证了哪个?桓杩汥慬????????????????????????楦浲獵??‰???堠湡桴浯湯獡？？

简介：Thispaperanalysesthefiveyears'monitoredstrainscollectedfromalong-termhealthmonitoringsysteminstalledonabridgewithwavelettransform.Intheanalysis,themonitoredstrainsarepre-processed,featuresofthemonitoreddataaresummarizedbriefly.Theinfluencesofthebasefunctionsontheresultsofwaveletanalysisarestudiedsimultaneously.Theresultsshowthatthedbwaveletisagoodmotherwaveletfunctionintheanalysis,andtheorderNshouldbelargerthan20,butlessthan46indeco...

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简介：Neisseriameningitidis是侵略meningococcal疾病的代理人，包括服的脑膜炎和败血病。因为不同的同种细胞的组(顺序类型)引起的疾病有他们的自己的流行病学的特征，在N的染色体之中理解差别是重要的。meningitidis同种细胞的组。到这个目的，染色体的一个结构的点阴谋的新奇解释被设计并且适用；在N的染色体之间的准确核苷酸火柴。meningitidisserogroupA紧张Z2491和serogroupB紧张MC58被识别，导致各种各样的结构的区域的说明。知道并且为每N的通常认为的毒力基因。meningitidis紧张然后被分类进这些区域。我们比Z2491做那些发现MC58的毒力基因更趋于到translocated区域(在新顺序上下文的chromosomal片断)，显著地向在translocated区域和在同一直线上的区域之一之间的接口趋于。在在同一直线上的区域以内，毒力基因趋于在准确火柴发生在差距的16kb以内。用在每秒字符数地点聚类的基因的这些趋势的确认是足够地支持的建议这些趋势能被用来集中搜索为并且在这二个有机体毒力基因和致病力的机制理解。

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简介：InordertorevealvariationandrevolutionofNPgenesofhumanavianH_5N_1influenzavirusstrains,theNPgeneofahumanavianH_5N_1influenzavirusstraininGuangdongwassequencedandtheglobalNPgenesofstrainswereretrieved.ThesequenceswereanalyzedbyDNAStar5.0,andtheevolu-tionaryspeedwasstudiedwithreferencetotheepidemiologicaldata.ItwasfoundthatNPgenesof45strainsduring1997-2006werehomologicallyclassifiedintothreegroups:strainsin1997-1998,strainsin2004-2005andstrainsfrom2003to2006.Therewere35substitutionsinNPsinallstrainsaccountingforaratioof7.03%(35/498).Anadditionalglycoproteindomain(NGT_(430-432))wasfoundinNPgenesinthestrainsof2003-2006,themutationofN_(370)SinGD-01-06resultedinoccurrenceofonemoreglyco-proteindomain(NES_(368-370)).Inthesynonymousvariation,K_svaluesinNPwere2.03×10~(-5)-2.55×10~(-5)Nt/dandK_avaluesinNPwere1.58×10(-6)-3.10×10~(-6)Nt/d.Theredidn′texistobviouslyselectivepres-sure.Anadditionalglycoproteindomainineverystrainof2003-2006andonemoreinstrainGD-01-06mightchangetheantigenicityofhumanavianH_6N_1influenzavirus.ThevariationonhumanavianH_5N_1influenzastrainsoccurredfrequentlyinthenaturalworld,whichwouldresultinhighprobabilityofhu-man-humantransmissionalongwiththenaturalevolutionofthevirus.

简介：农业上的基因操作重要昆虫现在为更高度有效的生物学上基于的人口控制程序允许基因sexing和男绝育系统的开发，最尤其是无菌的昆虫技术(坐)，为植物和动物昆虫害虫。四圜素能压制住(Tet离开)有条件的致命的系统可以一起工作以便转基因的种类将是在包含四圜素的节食可行、肥沃，却女性致命、男性处于没有四圜素的条件无菌。这将为无菌的男性唯一的生产在一个统一系统允许他们的很有效的使用为坐。然而，为如此的转基因的昆虫紧张的地版本的批评考虑是频率和致命性revertant幸存的基因基础的一颗决心。这将提供对评估致命性系统的基因稳定性必要的知识，它的环境安全，并且为保证最佳的功效的修正提供基础。为Tet离开致命的幸存决心，大规模屏蔽协议的开发应该也允许测试这些修正，并且测试另外的有条件的致命的系统的能力充分压制繁殖稀罕Tet离开幸存者。如果主导的温度敏感(DTS)蛹的致命性系统在果蝇为第二等的致命性证明有效，它可以提供需要支持sexing/sterility紧张的版本的保护措施，并且潜在地，版本不分男女致命性作为基因男绝育的一张表格拉紧。假如DTSProsβ21变化应该为冗余的致命性证明有效，它结构、功能的保存的高水平应该允许主人特定的cognates为大量昆虫种类被创造。

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简介：ToelucidatethestructuresofSLA-DR(swineleukocyteantigenDR)genesofthreeChinesepigstrains(Gz,BmandYn),theSLA-DRAandSLA-DRBcDNAwereamplifiedbyRT-PCRandsubjectedtodeterminethesequences.ThewholestructuresofSLA-DRAallelesareidenticalamongthreestrains,consistingof759nucleotidesincludinganopenreadingframe(ORF),andaresharedwiththosereportedfromNIHminipigsSLA-DRAcandSLA-DRAd.ThesamelengthoftheORF-containingSLA-DRBgenesofthreeChinesepigstrainswasalsoidentified.Theyarecomposedof801nucleotidesencodingaxenogeneicantigenmoleculeof266aminoacidresidues.ThenucleotidesequencesoftheSLA-DRBgenes,however,aredifferentwhencomparedeitheramongthethreestrainsorwiththepublisheddataofSLA-DRBsequences,whichallowedournovelSLA-DRBallelesreceivingtheiraccessionnumbersAY102479,AY102480andAY102481fromtheGenBank.ThisstudyfurtherrevealsthatthephylogenichomologiesofMHCDRorDR-likegenesinstructuresofnucleotidesanddeducedaminoacidsbetweenChinesepigs(SLA)andhuman(HLA-DRB1*0901)arebetterthanthosebetweenpigsandmice(H-2bEβ).HighsimilaritieswerealsofoundforDRα-DRβheterodimersbetweenChinesepigsandhumanintermsofaminoacidssequencescriticalforbindingwithhumanCD4coreceptormolecule,whicharebetterthanthosebetweenSLA-DRandH-2I-Emolecules.Afunctionaltestindicatedthat,bycotransfectionwithBm-DRAandBm-DRBgenes,theBm-DRmolecule-expressedL929cellscouldstimulatehumanTcellsquitewellinaxenogeneicreactioninpresenceofhumanAPCs.

简介：临床的试用和动物实验的增加的数字显示出细菌为过敏症预防正在答应工具的那probiotic。这里，我们在过敏促进感受性上分析了三选择乳杆菌紧张的immunomodulatory性质和他们的混合的影响赌1用一只gnotobiotic老鼠建模的v。我们显示出那乳杆菌(L.)rhamnosusLOCK0900，L。rhamnosusLOCK0908和L。caseiLOCK0919经由像使用费的受体被认出2(TLR2)并且核苷酸绑定oligomerization包含域的蛋白质2(NOD2)受体并且刺激骨头在种类依赖者和紧张依赖者礼貌生产cytokines的导出髓的树枝状的房间。殖民无菌(GF)有所有三紧张(Lmix)的混合物的鼠标由加强enterocytes的顶端的junctional建筑群并且恢复延续到终端网的微细丝的结构改进了肠的障碍。与Lmix开拓殖民地于并且敏化1变应原到赌注v的老鼠显著地象一个增加的转变生长因素(TGF)一样在sera和肠的lavages显示出变应原特定的IgE，IgG1和IgG2a和提高的全部的IgA水平的底层-β；与敏化的GF老鼠相比铺平。从开拓殖民地于Lmix的老鼠的Splenocytes和mesenteric淋巴节点房间显示出TGF-β的重要upregulation；在以后在有赌注v的vitro刺激1。我们的结果证明Lmix殖民改进了内脏对赌注v的上皮的障碍和减少的过敏促进感受性1。而且，这些调查结果被传播和能分泌的IgA和规章的cytokineTGF-β的增加的生产伴随；。因此，三乳杆菌紧张的这混合物在人在增加的内脏渗透和过敏症的发作的预防为使用显示出潜力。